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2654 次 Trabecular cell monolayer culture 2011-9-2
Trabecular cell monolayer cultureOriginally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
2430 次 Isolation of rodent pancreatic β cells 2011-8-1
Isolation of rodent pancreatic β cells 1.Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2.Rat islets were isolated from mal
2500 次 Culture of human renal proximal tubule cells and renal cortical fibroblasts 2011-7-11
Culture of human renal proximal tubule cells and renal cortical fibroblasts 1.The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibrobla
2243 次 Isolation of human colonic epithelial cells (EC) 2011-6-21
Isolation of human colonic epithelial cells (EC)1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were
2430 次 Primary brain cell isolation and culture 2011-6-13
Primary brain cell isolation and culture. 1.Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containin
4069 次 xCELLigence系統實時監測低毒性X-tremeGENE DNA轉染實驗 2011-6-10
xCELLigence系統實時監測低毒性X-tremeGENE DNA轉染實驗前言X-tremeGENE DNA Transfection Reagent是一種非脂質體、多組分轉染試劑，已被證明可有效轉染多種細胞。X-tremeGENE 9和X-tremeGENE H
3289 次滲透脅迫后擬南芥根表皮細胞膨壓恢復中離子吸收的作用 2011-5-6
滲透脅迫后擬南芥根表皮細胞膨壓恢復中離子吸收的作用注：滲透脅迫誘導的細胞膨壓和K+離子流的動力學變化。高滲處理導致膨壓快速下降，同時K+內流增加，膨壓在40min時恢復，K+內流減小。提高
2412 次 Measurement of primary endothelial cell permeability to fluxes of dextran or albumin 2011-4-1
Measurement of primary endothelial cell permeability to fluxes of dextran or albuminThe fluxes of albumin or dextran across vascular endothelial cell (ECs) were evaluated using Tra
15282 次 Isolation and Culture of Human Brain Tumor Stem Cell 2011-4-1
Isolation and Culture of Human Brain Tumor Stem CellThe isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes h
2534 次 Identification and expansion of the tumorigenic lung cancer stem cell population 2011-4-1
Identification and expansion of the tumorigenic lung cancer stem cell populationLung cancer contains a rare population of CD133+ cancer stem-like cells able to self-renew and gener
2545 次 Isolation and Culture of Human Liver Stem Cells 2011-4-1
Isolation and Culture of Human Liver Stem CellsMaterials and Methods1.Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy li
2291 次 FITC標記抗體-Marsshall氏法 2010-10-13
FITC標記抗體-Marsshall氏法材料：抗體球蛋白溶液、0.5mol/L pH9.0碳酸鹽緩沖液、無菌生理鹽水、異硫氰酸熒光素、1%硫柳汞水溶液、三角燒瓶（25—50ml）、冰及冰槽（或1000ml燒杯）、電磁攪拌器
4150 次 xCELLigence 系統內源性GPCRs 細胞功能研究 2010-10-11
xCELLigence系統實時評測內源性G-偶聯蛋白受體（GPCRs）功能Jeff Irelan 美國圣地亞哥ACEA Biosciences 公司Jonathan H. Morgan 美國弗吉尼亞州Manassas ATCC 產品經理前言 G-偶聯蛋白受體（GPC
3134 次原代細胞骨架的染色方法 2010-10-11
原代細胞骨架的染色方法微絲的顯示方法步驟：1. 用PBS液漂洗蓋片培養的原代細胞3次，每次30s；2. 用2%的甲醛/PBS液固定原代細胞3min；3. 用0.5%的三硝基甲苯/PBS處理3次，每次10min；4. PBS漂洗
3035 次原代細胞富爾根（Feulgen）反應顯示DNA 2010-10-11
原代細胞富爾根（Feulgen）反應顯示DNA基本原理：顯示DNA的傳統方法是富爾根（Feulgen）反應，切片先用稀鹽酸處理，DNA經弱酸（1mol/L HCL）水解后，在60℃條件下使DNA分子中脫氧核糖與嘌呤之間
6634 次 xCELLigence系統實時檢測神經毒性 2010-10-11
xCELLigence系統持續檢測神經細胞培養Sebastian Diemert, Julia Grohm, Svenja,Tobaben, Amalia Dolga, Carsten Culmsee德國，馬爾堡大學，藥理學與臨床藥學研究所摘要：為了研究類神經元細胞H
2742 次原代細胞DNA與RNA的吖啶橙熒光染色法 2010-10-9
原代細胞DNA與RNA的吖啶橙熒光染色法基本原理：吖啶橙（acridine orange，AO）是一種常用熒光染料，吖啶橙與原代細胞內的DNA、RNA都有親和力，但有一定的特異性，即結合后發不同顏色的熒光，DNA
2359 次原代細胞甲基綠-哌咯寧法顯示DNA和RNA 2010-10-9
原代細胞甲基綠-哌咯寧法顯示DNA和RNA基本原理：甲基綠（methyl green）和哌咯寧（pyronin）均為堿性染料，因此可與帶負電荷的磷酸根形成鹽。甲基綠分子有2個正電荷，易與雙鏈DNA分子結合使原代
3454 次原代細胞的脂類染色方法蘇丹紅Ⅲ染色法 2010-9-26
原代細胞的脂類染色方法蘇丹紅Ⅲ染色法原理：蘇丹紅染料在脂類物質中的溶解度大于其在溶劑中的溶解度，如果染料與含有脂類的試樣接觸，便有大量染料進入脂類物質的結構內，使這些結構呈紅色。多
4348 次蘇木精-伊紅（HE）染色 2010-9-26
蘇木精-伊紅（HE）染色染液制備：1. 蘇木精染液（1）稱取0.5g蘇木精、5.0g銨礬或鉀礬和0.1g碘酸鈉加溫溶于70ml蒸餾水中；（2）加入30ml甘油和2ml冰乙酸充分混勻后過濾即成母液；（3）母液可長