電泳時(shí)的DNA分子量Marker和Agarose濃度的選擇
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電泳時(shí)的DNA分子量Marker和Agarose濃度的選擇
|
DNA片段長(zhǎng)度 |
Agarose濃度 |
使用Marker種類 |
|
200 bp以下 |
3%以上 |
фX174-Hae Ⅲ digest DNA Marker
фX174-Hinc Ⅱ digest DNA Marker
DL500™DNA Marker
DL1,000™DNA Marker
DL2,000™DNA Marker
20 bp DNA Ladder Marker
50 bp DNA Ladder Marker
100 bp DNA Ladder Marker
150 bp DNA Ladder Marker
Wide Range DNA Marker (100~6,000) |
|
200 bp~700 bp |
2%~3% |
фX174-Hae Ⅲ digest DNA Marker
фX174-Hinc Ⅱ digest DNA Marker
DL500™ DNA Marker
DL1,000™ DNA Marker
DL2,000™ DNA Marker
DL5,000™ DNA Marker
50 bp DNA Ladder Marker
100 bp DNA Ladder Marker
150 bp DNA Ladder Marker
200 bp DNA Ladder Marker
250 bp DNA Ladder Marker
500 bp DNA Ladder Marker
Wide Range DNA Marker (100~6,000) |
|
700 bp~1,500 bp |
1%~2% |
λ-EcoT14Ⅰdigest DNA Marker
фX174-Hae Ⅲ digest DNA Marker
фX174-Hinc Ⅱ digest DNA Marker
DL2,000™ DNA Marker
DL5,000™ DNA Marker
DL10,000™ DNA Marker
50 bp DNA Ladder Marker
100 bp DNA Ladder Marker
150 bp DNA Ladder Marker
200 bp DNA Ladder Marker
250 bp DNA Ladder Marker
500 bp DNA Ladder Marker
Wide Range DNA Marker (100~6,000)
Wide Range DNA Marker (500~12,000)
Wide Range DNA Marker (500~15,000) |
|
1,500 bp~5,000 bp |
0.7%~1% |
λ-EcoT14Ⅰdigest DNA Marker
λ-Hind Ⅲ digest DNA Marker
DL5,000™ DNA Marker
DL10,000™ DNA Marker
DL15,000™ DNA Marker
Supercoiled DNA Ladder Marker
200 bp DNA Ladder Marker
250 bp DNA Ladder Marker
500 bp DNA Ladder Marker
Wide Range DNA Marker (100~6,000)
Wide Range DNA Marker (500~12,000)
Wide Range DNA Marker (500~15,000)
1 Kbp DNA Ladder Marker |
|
5,000 bp以上 |
0.7%以下 |
λ-EcoT14Ⅰdigest DNA Marker
λ-Hind Ⅲ digest DNA Marker
DL10,000™ DNA Marker
DL15,000™ DNA Marker
Supercoiled DNA Ladder Marker
Wide Range DNA Marker (500~12,000)
Wide Range DNA Marker (500~15,000)
1 Kbp DNA Ladder Marker |
