高表達vimentin的巨噬細胞在肝細胞癌免疫抑制中的關鍵作用
Vimentin結構. Schematic representation of a vimentin filament from a monomer to its assembly into a mature filament. The vimentin monomer is composed of a central a-helical domain flanked by an unstructured head and tail domain. On the central domain, vimentin possesses a rod II domain with lectin-like properties.
重要發(fā)現(xiàn)：Vimentin高表達（VIM+）巨噬細胞通過分泌IL-1β激活Treg細胞的免疫抑制功能
復旦大學的王紅陽/陳磊教授團隊于2024年9月在Nature Cancer （IF：23.5）上發(fā)表了題為“Spatial single-cell protein landscape reveals vimentinhigh macrophages as immune-suppressive in the microenvironment of hepatocellular carcinoma”的研究性文章。該文繪制了HCC單細胞空間蛋白圖譜，解析了HCC腫瘤間空間異質性，建立了預后相關的干細胞癌（HCC）空間分型（Spatial Pattern），并整合多組學和體外實驗揭示了Vimentin高表達的巨噬細胞與Treg細胞的空間互作促進HCC的疾病進展。
該項研究通過開展了單細胞測序和空間轉錄組分析，揭示了VIM+巨噬細胞的基因表達特征，發(fā)現(xiàn)了VIM+巨噬細胞和Treg之間基因表達的高度關聯(lián)性，提示了VIM+巨噬細胞可能作用于Treg，從而增強免疫抑制。配受體分析進一步揭示了VIM+巨噬細胞或許通過白介素-1β（IL-1β）和干擾素-γ（IFN-γ）參與對Treg細胞的調控。隨后，作者利用流式細胞儀分離了VIM+和VIM低表達的巨噬細胞，并檢測了其中IL-1β的表達水平。結果表明，在不同類型的巨噬細胞中，VIM+的巨噬細胞顯著高表達IL-1β，細胞共培養(yǎng)實驗發(fā)現(xiàn)了VIM+巨噬細胞促進Treg分泌IL-10，而加入IL-1β抗體可顯著緩解作用效果。
斯達特S-RMab®
Vimentin Recombinant Rabbit mAb (SDT-029-120) （貨號：S0B2254）抗體是您Vimentin 研究中的有力幫手。
數(shù)據(jù)驗證：
WB免疫印記
WB result of Vimentin Rabbit mAb
Primary antibody: Vimentin Rabbit mAb at 1/500 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 57 kDa
IHC 免疫組化
斯達特S-RMab® 系列抗體賦能生命科學和病理研究
免疫細胞化學
ICC shows positive staining in HeLa cells. Anti-Vimentin antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
產(chǎn)品信息
杭州斯達特 (www.starter-bio.com)志在為全球生命科學行業(yè)提供優(yōu)質的抗體、蛋白、試劑盒等產(chǎn)品及研發(fā)服務。依托多個開發(fā)平臺：重組兔單抗、重組鼠單抗、快速鼠單抗、重組蛋白開發(fā)平臺（E.coli,CHO,HEK293,InsectCells）,已正式通過歐盟98/79/EC認證、ISO9001認證、ISO13485。
