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          Rab11-GTP 小鼠單抗實驗使用及操作手冊

          瀏覽次數(shù):1347 發(fā)布日期:2023-3-16  來源:費斯德生物官網(wǎng)
          Product Description
          Pioneering GTPase and Oncogene Product Development since 2010
          Rab11-GTP
          Catalog Number: 26919
          Gene Symbol: RAB11
          Description: Anti-Rab11-GTP Mouse Monoclonal Antibody
          Background: Small GTPases of the Rab-family are crucial elements of the machinery that controls
          membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.
          Immunogen: Recombinant full length protein of active
          Rab11
          Tested Applications: IP, IHC
          Recommended Dilutions:
          IP: 1 g for 1~2 mg total cellular proteins.
          Immunoprecipitation and Western blot:
          IHC: 1:50~100 dilution
          Concentration: 1 mg/ml
          Host: Mouse
          Clonality: Monoclonal
          Isotype: IgG2a, IgG2b P
          Purity: Purified from ascites
          Format: Liquid
          Preservative: No
          2+2+
          Constituents: PBS (without Mg and Ca ), pH 7.4, 150 mM NaCl, 50% glycerol
          Species Reactivity: Anti- Rab11-GTP antibody
          recognizes active Rab11 from vertebrates.
          Storage Conditions: Store at -20°C. Avoid freeze /
          thaw cycles
          Rab11 Activation Assay.
          Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPS (lane right). Immunoprecipitation
          was done with the anti-Rab11-GTP monoclonal antibody (Cat. No. 26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21157).
          FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC APPLICATIONS
           
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