ProcartaPlex 免疫檢測常見問題及解決方案

eBioscience公司Luminex液相蛋白芯片檢測試劑——ProcartaPlex可以為您的生物學研究提供各種多因子檢測方案，包括細胞因子、生長因子、趨化因子和其他蛋白。在選擇試劑盒及操作時會遇到很多問題，現將常見問題及答復匯總如下，供您參考：

如果不小心將整個試劑盒保存在-20°C. 這個試劑盒還能再用嗎？

I accidentally stored the whole kit at -20°C. Can I use it anyway?

A.    試劑盒中除微球外，其余都可以保存在-20oC。如果微球在0oC以下保存，會損傷微球使得微球不能再用了。

All the components included in the ProcartaPlex kit are viable except the bead mixture. Storage of the beads at temperatures below 0oC will damage the beads and render them unusable.

如果我同時組合購買了多因子試劑盒和單因子試劑盒，還需要再買基礎試劑盒嗎？

Do I need to purchase a Basic Kit when combining Simplex Kits with a Multiplex Kit?

A.    不需要。

No, when combining a Multiplex kit with Simplex Bead Sets, the Basic Kit is not required. All the non target specific reagents which are included in the Basic kit are components of our Multiplex kits. Only when you are combining multiple Simplex kits (i.e. no Multiplex kit in the panel) will you need to also purchase the Basic Kit.

我能否將小鼠基礎試劑盒與人的單因子檢測試劑盒搭配使用？

Can I use the ProcartaPlex Mouse Basic Kit with ProcartaPlex Human Simplex and vice versa?

A.    不能。基礎試劑盒有種屬特異性。不同種屬的基礎試劑盒之間不能交叉使用。

No, the Basic Kits are specific to the species listed, i.e. the Mouse Basic Kit can only be used with Mouse Simplex Bead Sets. The Mouse, Human, Canine, Porcine, Rat and NHP ProcartaPlex Basic Kits are not interchangeable.

多因子檢測試劑盒不是同時購買的，可以搭配使用嗎？

Can I combine ProcartaPlex kits, which were not purchased on the same order? For example: I purchased a kit 3 months ago and another kit today. Can I combine them or do the kits have to be shipped on the same order?

A.    可以的。只要確保同時檢測的試劑盒的微球編號不重復，樣本前期處理相同即可。

Yes, you can combine ProcartaPlex kits from different orders to run a bigger multiplex experiment. You will want to ensure that there is no overlap of bead regions between the kits you are combining. Please refer to the online ProcartaPlex Panel Configurator.

Luminex的微球能在普通流式細胞儀上檢測嗎？

Is it possible to measure Luminex beads on a flow cytometer?

A.    不行。檢測ProcartaPlex (Luminex) 可以使用Luminex 100/200, MAGPIX, FLEXMAP 3D.

To analyze ProcartaPlex (Luminex) plates, you will need access to a Luminex machine such as a Luminex 100/200, MAGPIX, FLEXMAP 3D.

Luminex檢測時，建議收集多少微球？

How many beads do you recommend we collect? And how do we setup the Luminex machine?

A.    推薦每種微球收集50-100個。

Please collect 50-100 beads per bead region as stated in the product manual.

ProcartaPlex酶標板在信號不損失的情況下，是否可以多次上機檢測讀板？

Can a finished ProcartaPlex plate be read multiple times on the Luminex instrument without a significant signal loss?

A.    可以的。

Yes, it is possible to reread a finished ProcartaPlex plate without a loss in the signal or the number of beads counted. Please note that the Luminex instrument adds additional liquid to the wells with each analysis. It is possible that the wells may become overfilled with fluid after the third analysis. Hence, we do not recommend reading the ProcartaPlex plates more than two times.

如果ProcartaPlex操作完成，不能及時上機檢測。那我該如何保存？

I have completed the ProcartaPlex assay but can’t run my samples immediately. How long can I store the ProcartaPlex plate before analysis on a Luminex instrument without a significant loss of signal?

A.    正常情況，我們建議立即上機檢測；如果需要保存，建議在2-8oC保存。但是時間不能超過1天。

Ideally, we recommend that you analyze the samples immediately. If you do need to store your samples, overnight storage at 2-8°C shows no significant signal loss. We do not recommend storing the ProcartaPlex plate longer than 1 day.

洗滌步驟，我是否可以使用離心的方法操作？

Can I use a centrifuge for the washing steps in the assay?

A.    不可以。

No, we do not recommend centrifuging the magnetic beads for prolonged periods of time.

試劑盒中標準品溶解后，是否可以再保存使用？

Can I store reconstituted standard protein for later usage?

A.    不推薦。建議每次試驗使用新的標準品。

The recombinant standards included with the ProcartaPlex assay are one-time use standards. Once reconstituted for use, we do not recommend storing and reusing this recombinant standard. With each new experiment, we recommend reconstituting a fresh unused vial of recombinant standard protein.

標準品的初始溶解母液濃度從哪里得知？

Where can I find the stock concentration of the recombinant standards?

A.    請在隨貨收到的說明書中找到。

This information can be found on the Certificate of Analysis provided with the ProcartaPlex assay.

實驗中，標準曲線和樣本是否可以做單孔檢測？

When preparing standards/samples, can I run them as single points?

A.    為了獲得更好的結果準確性，我們建議樣本和標準曲線孔至少做復孔。

For better accuracy, we recommend that you run samples/standards as duplicates at a minimum.

如果我不得不中止實驗，我可以在哪步停止？

I have to cut short the assay and can’t complete the assay. At which step can we incubate the plates overnight with affecting the assay?

A.    按照說明書推薦，可以在加入標準品和樣本后過夜反應。

As stated in the product manual, after the addition of the standards and samples, the plate can be stored overnight (Step 11.1.5 or 11.2.5).

如果需要更多信息，請查看如下鏈接： http://us.ebioscience.com/resources/faq/procartaplex-multiplex-immunoassays

閱讀原文：http://www.liankebio.com/TechServiceShow/articleID/2014040017.html